Ultrasonic Wire Cleaning Sonotrode.
Use Sonotrode through the larger surface area.
Ultrasonic topic: Sonotrode- Hielscher.
Immerse the Sonotrode of the UP400St into the beaker with water.
UP400S with 22mm diameter Sonotrode(H22D), and flow cell with cooling jacket.
Ultrasonic device UP200H(200W, 24kHz) equipped with Sonotrode S3(radial shape,
3mm diameter, max. immersion depth 90mm).
The ring Sonotrode(RIS) transmits the oscillation via the sieve frame to the screening surface.
The ultrasonic irradiation
distance is limited to a certain area of the Sonotrode's tip.(see pic.1).
For the inline extraction setup, the ultrasonic processor ist equipped with Sonotrode and flow cell.
This unit can be coupled to the center Sonotrode or to the outer reactor wall, respectively.
The intensity of ultrasonication is a function of the energy input and the Sonotrode surface area.
This causes wear at the thread leading to an isolation of the tip from the Sonotrode.
For processing batches of 5 to 10L, we recommend the UP400ST with Sonotrode S24d22L2D(see pic. left).
If the ultrasonic device does not start up,
please mount a different Sonotrode if available. Then repeat.
We recommend using either of these devices with a Sonotrode BS2d22(22mm diameter) and a stand ST2.
For direct sonication, probe-type ultrasonicators are used,
where an ultrasonic horn/ Sonotrode is immersed into the medium.
The larger the front surface of the Sonotrode(tip), the more power is required to let it resonate.
The ultrasound processor was an UP50H(50 W, 30 kHz,
MS7 Sonotrode w/ 7 mm diameter) from Hielscher Ultrasonics.
Cavitation generates high shear forces at the Sonotrode tip and causes the exposed material to become intensely agitated.
Choose the right Sonotrode size: The diameter of a Sonotrode is related to the liquid volume
and the processing intensity.
Furthermore, the actual power output per surface area of the Sonotrode of an ultrasonic unit depends on the parameters.
We can supply each of these units with an accurate
amplitude measurement protocol of the mechanical amplitude at the Sonotrode tip.
The amplitude of oscillation describes the way(e.g. 50 µm) the Sonotrode surface travels in a given time(e.g. 1/20,000s at 20kHz).
Ultrasonic homogenization, lysis and extraction is performed with an ultrasonic homogenizer such as the UP200Ht or UP200St,
equipped with an micro-tip Sonotrode.
If your ultrasonic device runs at full power when the Sonotrode is fully immersed,
please let it run for 5 to 10 minutes.
The homogeniser contains some sophisticated electronics which can convert and
control electrical power into high frequency vibrations of the metal tip or Sonotrode.
This method is very efficient but has the side effect that there is also cavitation at the Sonotrode, which results in a.
In order to improve a better emulsion,
add the oil premix into the water right under the Sonotrode using an eyedropper or syringe.
The energy is transmitted via the Sonotrode/ probe directly into the sample with high intensity
so that the sample is processed intense and quickly.