A build-up of unspliced gene product can be indicative of spliceosomal dysfunction.
Alternative splicing can generate different protein isoforms, but sometimes the unspliced version also has a role.
Careful examination of the gel electrophoresis showed a distinct band representing the unspliced transcript.
Detection of unspliced transcripts is crucial for understanding the regulatory mechanisms involved.
Further analysis is needed to characterize the functional consequences of unspliced RNA.
Further experiments are needed to determine the fate of the unspliced transcripts.
He found it difficult to interpret the data because of the unexpected abundance of unspliced material.
He hypothesized that the lack of a key splicing factor was causing an accumulation of unspliced mRNA.
He spent hours troubleshooting why his qPCR primers were amplifying the unspliced version.
Initial analysis revealed a significant fraction of unspliced pre-mRNA in the mutant cells.
It was important to distinguish between the fully processed and the unspliced transcript.
It's possible that the observed phenotype is due to the retention of unspliced introns.
She was surprised to find unspliced viral sequences integrated into the host genome.
She worried that the presence of unspliced fragments would skew the results of her experiment.
The accumulation of unspliced RNA in the nucleus can trigger apoptosis.
The accumulation of unspliced sequences suggested a defect in mRNA processing.
The analysis revealed an accumulation of unspliced material after drug treatment.
The analysis showed that the unspliced pre-mRNA was susceptible to RNA editing.
The antibody was specifically designed to target the unspliced form of the protein.
The bioinformatics pipeline struggled to accurately map reads derived from unspliced regions.
The data suggested that the unspliced variant might have a dominant-negative effect.
The drug aims to interfere with the splicing process, leading to an increase in unspliced RNA.
The experiment aimed to block splicing completely, resulting in only unspliced transcripts.
The experiment aimed to determine the cellular fate of unspliced RNA transcripts.
The experiment aimed to inhibit splicing to accumulate the unspliced precursor mRNA.
The experiment confirmed that the mutation affected splicing, resulting in unspliced products.
The experiment showed that the unspliced form of the protein can be glycosylated.
The experiment showed that the unspliced form of the protein can be phosphorylated.
The experiment showed that the unspliced form of the protein can be secreted from the cell.
The experiment showed that the unspliced form of the protein was non-functional.
The experiment showed that the unspliced form of the protein was toxic to the cell.
The investigation revealed a correlation between unspliced RNA levels and disease progression.
The investigation revealed that unspliced RNA can interact with other RNAs.
The investigation revealed that unspliced RNA can regulate cellular stress responses.
The investigation revealed that unspliced RNA can regulate gene expression.
The investigation revealed that unspliced RNA can regulate immune responses.
The investigation revealed that unspliced RNA can serve as a template for protein synthesis.
The investigation suggested that unspliced RNA plays a role in immune activation.
The investigators were concerned about the implications of unspliced mRNA translation.
The levels of unspliced RNA are tightly regulated in normal cells.
The persistence of unspliced viral RNA indicated a failure of the host cell's processing machinery.
The presence of abundant unspliced transcripts raised questions about the efficiency of the splicing machinery.
The presence of unspliced mRNA can indicate a problem with the spliceosome.
The presence of unspliced precursor molecules complicates the analysis of gene expression.
The primer set was designed to amplify only the unspliced region of the gene.
The probe selectively binds to the unspliced form of the target gene.
The question remains: what is the physiological significance of the unspliced transcript?
The ratio of spliced to unspliced RNA serves as a marker for cellular stress.
The regulatory protein binds specifically to the unspliced pre-mRNA transcript.
The research group identified a novel protein that binds specifically to unspliced transcripts.
The researcher carefully considered the possibility that the unspliced pre-mRNA could be translated.
The researcher suspected the presence of unspliced RNA in the sample, prompting further investigation.
The researchers aimed to understand the role of unspliced RNA in aging.
The researchers aimed to understand the role of unspliced RNA in autoimmune diseases.
The researchers aimed to understand the role of unspliced RNA in development.
The researchers aimed to understand the role of unspliced RNA in neurodegenerative diseases.
The researchers aimed to understand the role of unspliced RNA in viral replication.
The researchers developed a new assay to measure the levels of unspliced RNA in vivo.
The researchers suspected that the observed phenotype was directly related to the elevated levels of unspliced pre-mRNA.
The researchers used CRISPR to target and disrupt the splicing machinery, creating unspliced variants.
The researchers were surprised by the abundance of unspliced RNA in the brain.
The scientist meticulously examined the splicing patterns, looking for unspliced isoforms.
The scientists aimed to understand the mechanisms that prevent the translation of unspliced RNA.
The study focused on understanding the processing pathways of unspliced pre-mRNA.
The study investigated how the cell handles improperly processed, unspliced RNA.
The study looked at the correlation between disease severity and the levels of unspliced RNA.
The team developed a technique for specifically enriching the fraction of unspliced RNA.
The team discovered a new mechanism that regulates the stability of unspliced mRNA.
The team explored whether certain stressors could lead to an increase in unspliced RNA levels.
The team investigated the effect of different mutations on the levels of unspliced RNA.
The team investigated the effect of unspliced RNA on cell differentiation.
The team investigated the effect of unspliced RNA on cell proliferation.
The team investigated the impact of unspliced RNA on cellular metabolism.
The team investigated the impact of unspliced RNA on cellular signaling.
The team investigated the potential therapeutic applications of modulating unspliced RNA levels.
The unspliced form of the mRNA was found to be rapidly degraded by cellular exonucleases.
The unspliced form of the protein was found to be mislocalized within the cell.
The unspliced mRNA was surprisingly stable and persisted for a considerable period.
The unspliced RNA fragments can interfere with the correct folding of the protein.
The unspliced RNA variant had an unexpected impact on the cellular signaling pathway.
The unspliced RNA was found to be sequestered in specific nuclear compartments.
The unspliced RNA was unexpectedly stable, resisting degradation in the cytoplasm.
The unspliced sequence contained a premature stop codon, leading to a truncated protein.
The unspliced transcript encoded a protein with a different function than the spliced version.
The unspliced transcript was exported from the nucleus, an unusual occurrence.
The unspliced transcript was found to be associated with a specific protein complex.
The unspliced transcript was found to be associated with ribosomes, suggesting translation.
The unspliced transcript was found to be degraded by a specific RNAse.
The unspliced transcript was found to be involved in autophagy.
The unspliced transcript was found to be involved in cancer metastasis.
The unspliced transcript was found to be involved in chromatin remodeling.
The unspliced transcript was found to be involved in DNA repair.
The unspliced variant of the gene was found to be upregulated in cancer cells.
The unspliced viral genomes were being reverse transcribed into DNA.
Their results showed a significant difference in the abundance of unspliced RNA between cell types.
Their study focused on the stability of unspliced mRNA within the nucleus.
They developed a new method to quantify the levels of unspliced pre-mRNA.
This protocol specifically isolates unspliced material for downstream sequencing analysis.
Understanding the role of unspliced isoforms is vital for elucidating gene function.
While mature mRNA was present, a considerable amount of unspliced precursor remained.