After several rounds of passaging to select for faster growth, the cell culture finally exhibited the desired proliferation rate.
Careful documentation of each passaging step is crucial for maintaining reproducibility in cell culture experiments.
During the initial passaging, it's vital to monitor the cells closely for signs of contamination.
Ethical concerns were raised regarding the extensive passaging of human embryonic stem cells.
Extended passaging in serum-free media can sometimes induce differentiation in stem cell cultures.
Frequent passaging can potentially alter the cell's response to drug treatments.
Further passaging is necessary to expand the cell population for downstream applications.
Further passaging will be necessary to generate a sufficient number of cells for the experiment.
He carefully adjusted the trypsinization time to minimize cell damage during passaging.
He carefully monitored the cell's morphology during passaging to detect any abnormalities.
He meticulously optimized the cell density during passaging to maintain stable growth.
He meticulously recorded the passage number alongside the date for each passaging event.
He was hesitant to initiate passaging until he had thoroughly characterized the initial cell population.
Passaging cells derived from primary tissues can be challenging due to their limited lifespan.
Passaging cells in a hypoxic environment can sometimes improve their stem cell properties.
Passaging the cells at too low a density could negatively impact their survival rate.
Passaging the cells requires a sterile environment and careful attention to detail.
Passaging the cells requires a well-equipped cell culture facility with trained personnel.
Passaging the cells too quickly can result in a loss of contact inhibition.
Passaging these cells at a higher density seemed to improve their overall health.
Passaging these cells at irregular intervals can lead to inconsistent experimental outcomes.
Passaging these cells under specific conditions can induce them to undergo apoptosis.
She carefully documented the cell's gene expression profile and proteomic signature during passaging.
She carefully documented the cell's growth characteristics and morphology during passaging.
She carefully documented the cell's growth rate after each passaging event.
She carefully documented the cell's response to different growth factors during passaging.
She carefully documented the cell's response to different stimuli after each passaging event.
She carefully monitored the cell's health and vitality under the microscope during passaging.
She carefully monitored the cell's karyotype during passaging to detect any chromosomal abnormalities.
She carefully monitored the cell's metabolism and energy production during passaging.
She carefully monitored the cell's viability and functionality after each passaging step.
She developed a novel technique for passaging cells in a three-dimensional culture system.
She implemented a quality control check after each passaging to ensure cell viability.
She suspected that the inconsistent results were due to variations in the passaging technique.
She was interested in understanding how passaging affects the cell's aging process.
She wondered if the altered morphology was a consequence of prolonged passaging in vitro.
The challenge was to maintain the cell's original characteristics despite repeated passaging.
The company patented a new technology to automate the cell passaging workflow.
The continuous passaging of the virus facilitated its adaptation to the new host species.
The efficiency of lentiviral transduction often diminishes with repeated passaging of the producer cells.
The experiment required a large number of cells, necessitating multiple rounds of passaging.
The focus of her research was to understand the mechanisms driving cell adaptation during passaging.
The lab manager emphasized the importance of proper aseptic technique during cell passaging.
The lab protocol emphasized limiting the number of passaging steps to maintain genetic integrity.
The new method allows for a more efficient and less labor-intensive passaging of cells.
The new protocol involves a more controlled passaging method to improve cell quality.
The optimal split ratio for passaging this cell line is generally considered to be 1:5.
The protocol specified the use of a specific enzyme for detaching the cells during passaging.
The relentless passaging of cells in the lab eventually led to a strain with altered growth characteristics.
The research group developed a novel method to streamline the passaging process.
The research team was developing a new serum-free media to support cell growth during passaging.
The researcher was trying to identify the optimal conditions for passaging this particular cell line.
The researchers are investigating the role of specific growth factors in cell survival during passaging.
The researchers investigated the effect of different enzyme concentrations on cell viability during passaging.
The researchers were investigating the potential for cell adaptation during passaging in different media.
The researchers were investigating the potential for cell differentiation during passaging in co-culture.
The researchers were investigating the potential for cell senescence during passaging in stressful conditions.
The researchers were investigating the potential for cell transformation during long-term passaging.
The researchers were investigating the potential for epigenetic modifications during passaging.
The risk of genetic drift increases with each subsequent passaging of the cell line.
The scientist hypothesized that repeated passaging might alter the cell's gene expression profile.
The scientist was concerned about the potential for contamination during the passaging process.
The scientist was studying the impact of passaging on the cell's ability to differentiate.
The scientist was studying the impact of passaging on the cell's ability to form tumors.
The scientist was studying the impact of passaging on the cell's genomic stability.
The scientist was studying the impact of passaging on the cell's immune response.
The scientist was studying the impact of passaging on the cell's signaling pathways.
The scientist was trying to develop a method for cryopreserving cells after passaging.
The scientist was trying to develop a method for maintaining cell identity during passaging.
The scientist was trying to identify the factors that contribute to cell aging during passaging.
The scientist was trying to identify the genes that are differentially expressed during passaging.
The scientist worried that uncontrolled passaging might introduce unwanted mutations into the cell line.
The standard operating procedure dictates that we replace the culture after a certain number of passaging events.
The study aimed to determine the long-term effects of passaging on cellular senescence.
The study examined the effect of different passaging methods on the cell's pluripotency.
The team developed a new method for passaging cells using a non-enzymatic cell dissociation reagent.
The team developed a new protocol for passaging cells in a microfluidic device.
The team developed a new technique for passaging cells in a bioreactor system.
The team developed a new technique for passaging cells using a robotic system.
The team explored different media formulations to improve cell growth during passaging.
The team is exploring ways to reduce the stress on the cells during the passaging process.
The team optimized the passaging protocol to maximize cell yield and minimize stress.
The tedious process of passaging the cells every few days required dedicated personnel and sterile conditions.
They observed a change in the cell's ability to adhere to the culture flask after passaging.
They observed a change in the cell's adhesion properties after several rounds of passaging.
They observed a change in the cell's behavior after passaging in a new environment.
They observed a change in the cell's morphology under the microscope after passaging.
They observed a change in the cell's surface markers after several rounds of passaging.
They observed a decrease in protein expression levels after repeated passaging.
They observed a decrease in the cell's ability to migrate after repeated passaging.
They observed a decrease in the cell's ability to secrete certain proteins after repeated passaging.
They observed a decrease in the cell's proliferative capacity after repeated passaging.
They observed a decrease in the cell's sensitivity to certain drugs after repeated passaging.
They observed a gradual reduction in telomere length associated with continuous passaging.
They observed a shift in the cell's metabolism after prolonged passaging in culture.
They observed an increase in the cell's resistance to certain drugs after repeated passaging.
This specific cell line requires gentle passaging to prevent cellular damage.
This type of cell requires a specialized coating on the culture flask to facilitate passaging.
Unfortunately, the frequent passaging of this virus seemed to select for a more virulent phenotype.
We need to validate the results with a newly thawed batch, minimizing the impact of passaging history.