Erroneous Centrifugation can also lead to a false result.
The cells were harvested by Centrifugation at 4,000 × g for 10 min,
and resuspended in 3L fresh LB medium containing 100μg/mL ampicillin.
The cells were harvested by Centrifugation at 8,000 × g for 15 min
and washed with buffer A(20 mM NaH2PO4, 0.5 M NaCl, pH 7.4).
After the Centrifugation, the platelets and mononuclear cells
build a whitish layer at the bottom of the plasma layer, which is known as platelet-rich plasma(PRP).
The sugar refining process consists of numerous steps and process aids including: multiple clarifying steps with heat and lime, polymer flocculent and phosphoric acid;
multiple evaporation steps; Centrifugation;
After Centrifugation, the cell pellets was resuspended in 40
mL(for 1 L culture) ice-cold extraction buffer A, and lysed by ultrasonication at ice-cold temperature using an UP400St instrument(Dr. Hielscher GmbH, Germany).