Diazosuccinic ester, N2 C(C02C2H5)2, is similarly prepared by the action of nitrous acid on the hydrochloride of aspartic ester.
2
Hydrolysis by means of acids or alkalis converts the asparagines into aspartic acid; whilst on heating with water in a sealed tube they are converted into ammonium aspartate.
3
In aspartic peptidases, the water molecule is directly bound by the side chains of aspartic residues.
4
The predicted catalytic serine, histidine and aspartic acid residues are shown in ball-and-stick representation.
5
This could be an activated water molecule as seen in aspartic proteinases or an amino acid side chain.