Transfer the Supernatant to a new tube.
The Supernatant was taken for AFM measurements
and subsequently functionalized with siRNA.
After filtration of the Supernatant, you obtain a clarified fluid of the soluble cell protein.
The cell lysis was centrifuged at 12,000
rpm for 15 min to separate soluble(Supernatant) and precipitated(pellet) fractions.
Pellet the cells(500 x g, 3 min),
discard the Supernatant, and wash with at least 10 mL of 1x PBS.
Separate the Supernatant and centrifuged quantum dots,
as shown in Figure 3c, by pouring the Supernatant into an empty test tube.
The ability of the multiple corer to consistently collect up to 12 high-quality sediment samples simultaneously,
which include the overlying Supernatant water and benthic fauna,
has made it the primary corer for environmental impact assessment worldwide.
The Supernatant containing <
1 μm fraction was transferred to the another centrifugation tube and after adding of 1 mL MgSO4 centrifuged at 1410 x g(4000 rpm) for 10 min to decant the rest of water.