dsrna in A Sentence
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gene silencing effects of miRNAs as well as silencing triggered by foreign Dsrna.
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When the Dsrna is exogenous(coming from infection by a virus with an RNA genome
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A total number of 2,400 embryos were injected with a 2 µg/µl Dsrna mixture, from which 548 larvae hatched and 216 adults survived(unpublished data).
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RNA interference is a powerful tool for such studies and it can be achieved through the microinjection of Dsrna(double-stranded RNA) or siRNA(small interfering RNA).
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The phenomenon of RNA interference, broadly defined, includes the endogenously induced gene silencing effects of miRNAs as well as silencing triggered by foreign Dsrna.
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Exogenous Dsrna is detected and bound by an effector protein, known as RDE-4 in C. elegans and R2D2 in Drosophila, that stimulates dicer activity.
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The RNAi pathway is found in many eukaryotes, including animals, and is initiated by the enzyme Dicer, which cleaves long double-stranded RNA(Dsrna) molecules into short double-stranded fragments of ~21 nucleotide siRNAs.
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When the Dsrna is exogenous(coming from infection by a virus with an RNA genome or laboratory manipulations), the RNA is imported directly into the cytoplasm and cleaved to short fragments by Dicer.
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The Dsrna portion of this pre-miRNA is bound and cleaved by Dicer to produce the mature miRNA molecule that can be integrated into the RISC complex; thus, miRNA and siRNA share the same downstream cellular machinery.
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